pum1 primary antibody  (ABclonal Biotechnology)

Journal: Cell Death & Disease

Article Title: PUM1 knockdown prevents tumor progression by activating the PERK/eIF2/ATF4 signaling pathway in pancreatic adenocarcinoma cells

doi: 10.1038/s41419-019-1839-z

Figure Lengend Snippet: a PUM1 mRNA expression levels in PAAD tissues (T) and normal tissues (N) analyzed by gene expression profiling interactive analysis (GEPIA). * P < 0.05. b Analysis of the relationship between PUM1 mRNA expression and overall survival of PAAD patients analyzed using The Cancer Genome Atlas (TCGA)

Article Snippet: The PUM1 primary antibody was purchased from ABclonal Technology (Wuhan, China).

Techniques: Expressing, Gene Expression

Journal: Cell Death & Disease

Article Title: PUM1 knockdown prevents tumor progression by activating the PERK/eIF2/ATF4 signaling pathway in pancreatic adenocarcinoma cells

doi: 10.1038/s41419-019-1839-z

Figure Lengend Snippet: a Representative images of PUM1 expression by immunohistochemistry (IHC) analysis in tissue microarrays representing 60 PDAC patients. b Comparison of total scores for PUM1 assessment on tissue microarrays slides. Total score = positive staining scores × staining intensity score. * P < 0.05. c The overall survival rate of patients with PDAC was evaluated based on PUM1 expression using Kaplan–Meier analysis ( n = 60, P = 0.022)

Article Snippet: The PUM1 primary antibody was purchased from ABclonal Technology (Wuhan, China).

Techniques: Expressing, Immunohistochemistry, Comparison, Staining

Journal: Cell Death & Disease

Article Title: PUM1 knockdown prevents tumor progression by activating the PERK/eIF2/ATF4 signaling pathway in pancreatic adenocarcinoma cells

doi: 10.1038/s41419-019-1839-z

Figure Lengend Snippet: The relationship between PUM1 protein levels and clinic characteristics of patients with pancreatic adenocarcinoma

Article Snippet: The PUM1 primary antibody was purchased from ABclonal Technology (Wuhan, China).

Techniques: Expressing

Journal: Cell Death & Disease

Article Title: PUM1 knockdown prevents tumor progression by activating the PERK/eIF2/ATF4 signaling pathway in pancreatic adenocarcinoma cells

doi: 10.1038/s41419-019-1839-z

Figure Lengend Snippet: PUM1 mRNA ( a ) and protein ( b ) expression levels in PDAC cell lines (BxPC-3, AsPC-1, MIA PaCa-2, and PANC-1) and human pancreatic duct epithelial cells HPDE6-C7. c PUM1 expression levels in cells infected with lentivirus expressing the shRNA of PUM1 (shPUM1) or negative (NC) control empty lentivirus. d , e Effect of PUM1 knockdown on the OD values at 490 nm. f Effect of PUM1 knockdown on early apoptosis rate. b , c , f The representative graphs are on the left, and the statistical results are on the right. n = 3, * P < 0.05, for a – f

Article Snippet: The PUM1 primary antibody was purchased from ABclonal Technology (Wuhan, China).

Techniques: Expressing, Infection, shRNA, Control, Knockdown

Journal: Cell Death & Disease

Article Title: PUM1 knockdown prevents tumor progression by activating the PERK/eIF2/ATF4 signaling pathway in pancreatic adenocarcinoma cells

doi: 10.1038/s41419-019-1839-z

Figure Lengend Snippet: Effect of PUM1 knockdown on cell migration ( a ) and invasion ( b ) detected by Transwell assays. Representative graphs (left). Bar graph showing the statistical result of migratory or invasive cells (right). c Effect of PUM1 knockdown on the expression of MMP9, VEGF, vimentin, and E-cadherin. Representative graphs of western blotting (left). Relative protein expression in reference to GAPDH (right). n = 3, * P < 0.05, for a – c

Article Snippet: The PUM1 primary antibody was purchased from ABclonal Technology (Wuhan, China).

Techniques: Knockdown, Migration, Expressing, Western Blot

Journal: Cell Death & Disease

Article Title: PUM1 knockdown prevents tumor progression by activating the PERK/eIF2/ATF4 signaling pathway in pancreatic adenocarcinoma cells

doi: 10.1038/s41419-019-1839-z

Figure Lengend Snippet: a PUM1 expression levels in tumors formed by the shPUM1 or NC group cells. b , c Effect of PUM1 knockdown on the growth of subcutaneous xenograft tumor. b Pictures of subcutaneous xenograft tumor. c Tumor growth curve. n = 8, * P < 0.05. d , e Effect of PUM1 knockdown on lung metastases examined by hematoxylin and eosin staining, or Ki67 expression detected by immunohistochemistry in the lung tissues of a lung metastasis mouse model

Article Snippet: The PUM1 primary antibody was purchased from ABclonal Technology (Wuhan, China).

Techniques: Expressing, Knockdown, Staining, Immunohistochemistry

Journal: Cell Death & Disease

Article Title: PUM1 knockdown prevents tumor progression by activating the PERK/eIF2/ATF4 signaling pathway in pancreatic adenocarcinoma cells

doi: 10.1038/s41419-019-1839-z

Figure Lengend Snippet: Volcano plot ( a ), scatter plot ( b ), and cluster gram ( c ) of the cDNA microarrays. d Effect of PUM1 knockdown on the expression of key components of the eIF2 signaling pathway. Left: Representative graphs of western blotting. Right: Statistical result of the relative protein expression in reference to GAPDH. n = 3, * P < 0.05

Article Snippet: The PUM1 primary antibody was purchased from ABclonal Technology (Wuhan, China).

Techniques: Knockdown, Expressing, Western Blot

Journal: Cell Death & Disease

Article Title: PUM1 knockdown prevents tumor progression by activating the PERK/eIF2/ATF4 signaling pathway in pancreatic adenocarcinoma cells

doi: 10.1038/s41419-019-1839-z

Figure Lengend Snippet: a Representative pictures showing p-PERK levels in 60 PDAC tissues on tissue microarray examined by immunohistochemical analysis. b Linear regression analysis showing the correlation of PUM1 and p-PERK levels in PDAC tissues ( n = 60)

Article Snippet: The PUM1 primary antibody was purchased from ABclonal Technology (Wuhan, China).

Techniques: Microarray, Immunohistochemical staining

Journal: Biochemical and biophysical research communications

Article Title: Loss of preimplantation embryo resulting from a Pum1 gene trap mutation.

doi: 10.1016/j.bbrc.2015.04.019

Figure Lengend Snippet: Fig. 1. Generation of a murine Pum1 gene trap mutation. (A) Schematic representation of the gene trap insertion into the Pum1 gene between exon 2 and exon 3. Short linear bar and black bar show the b-geo probe and Pum1 probe used for Southern blot. Exons are represented as boxes. SA, splice acceptor; b-geo, b-galactosidaseeneomycin resistance cassette. (B) Southern blot containing DNA isolated from postnatal pups of heterozygous parents. DNA was digested with SacI or SphI and hybridized with the probes shown in A (black bar). The expected fragments generated by SacI are 4.9 kb for the wild-type allele, and 7.6 kb for the mutant allele. For SphI digestion, the expected fragments are 5.6 kb for the wild-type, and 8.9 kb for the mutant allele. b-geo probe recognized a 8.9 kbp fragment when digested with SphI. (C) Characterization of Pum1 mRNA transcript. Total RNA was extracted from adult testis (T), epididymis (E) and ovary (O) of wild-type and Pum1 heterozygous mice. Two different pairs of primers were used to amplify the mutant transcript. Wild-type and mutant RTPCR products were run side by side for each sample. b-actin was used as a control. (D) PCR genotyping analysis to distinguish wild-type allele (580 bp) and mutant allele (274 bp). (E) Confirmation of Pum1- bgeo fusion protein. Testis lysates from wild-type and heterozygous mice were first blotted with anti-Pum1 antibody, and then reprobed with b-geo antibody. The Pum1 antibody can only detect one band because the 127 kDa Pum1 protein co-migrates with the 129 kDa fusion protein.

Article Snippet: The blots were incubated in a 1:2000 dilution of the Primary anti-Pum1 antibody (Bethyl Lab) at 4 C overnight.

Techniques: Mutagenesis, Southern Blot, Isolation, Generated, Reverse Transcription Polymerase Chain Reaction, Control